An emergency lighting device with high safety includes a fixture base, two holders and a light tube. The holders are disposed on the fixture base. Each of the holder includes a holder body, two conductive plates and a rotational fixture. The conductive plates are disposed in the holder body and the rotational fixture is disposed in the central recess of the holder body. The light tube has two end caps and a tube body. The end caps are installed on the holders respectively. Each of the end caps has a non-conductive pin and two conductive pins inserted into the rotational fixture of one of the holders, such that the conductive pins of the end cap contact the conductive plates of the holder respectively.