A pixel circuit is disposed in the display substrate, the display substrate includes a display stage and a non-display stage, the pixel circuit is configured to drive the light emitting element to emit light in the display stage, and includes a first control sub-circuit, a second control sub-circuit, a third control sub-circuit, a fourth control sub-circuit, a light emitting control sub-circuit and a driving sub-circuit; the third control sub-circuit is electrically connected with a third reset signal terminal, a control signal terminal and a third node respectively, and is configured to provide a first signal to the third node in the display stage and a second signal to the third node or acquire a signal of the third node in the non-display stage under control of the third reset signal terminal.