The present application discloses a display panel and a preparation method thereof, and a display device. The display panel includes a base substrate, and has a display area, a peripheral area surrounding the display area, a hole area located at the display area, and an isolation area located between the hole area and the display area. The display area includes an electroluminescent device located on the base substrate, and a packaging structure for sealing the electroluminescent device, the packaging structure includes an inorganic packaging layer and an organic packaging layer alternately stacked, and the inorganic packaging layer extends to the hole area. The isolation area includes an organic layer located on the inorganic packaging layer.