A display substrate, including: a first film layer arranged on a side of a base substrate, a second film layer arranged on a side of the first film layer away from the base substrate and an adhesive material portion arranged therebetween; wherein the first film layer has a first surface and a second surface respectfully facing the second film layer and the first film layer, and the first surface is at least partially in contact with the second surface; the adhesive material portion is arranged at least partially in a non-flat contact area formed between the first film layer and the second film layer, to adhere the first film layer and the second film layer; an adhesion between the adhesive material portion and each of the first film layer and the second film layer is greater than an adhesion between the first film layer and the second film layer.