A particle sorting apparatus includes: a flow cell including a flow channel; an imaging element; and a controller. The controller performs a coarse adjustment onto a position of the flow cell based on an image regarding a flow axis of the flow channel and obtained by the imaging element, while continuously moving the flow cell at a first speed. The controller performs a final adjustment onto the position of the flow cell to allow the first intensity of the first light emitted from the light emitting object flowing in the flow channel to be maximum while moving the flow cell at a second speed that is lower than the first speed.