The gas detector calibration system comprises a control unit, a temperature regulation assembly, a pump, and a temperature sensor. The temperature regulation assembly has an inlet and an outlet, and the pump has a pump inlet and a pump outlet. The outlet of the temperature regulation assembly is coupled to the pump inlet of the pump. The temperature regulation assembly receives ambient air through the inlet, regulates a temperature of the ambient air, and supplies the ambient air to the pump through the outlet. The pump supplies the ambient air to a gas detector for regulating a temperature of the gas detector. The temperature sensor detects the temperature and sends a signal indicative of the temperature to the control unit. The control unit determines whether the temperature is within a predefined temperature range, when within the predefined temperature range, the gas detector is calibrated using a calibration gas.