Present disclosure relates a multi-factor authentication electronic lock, a multi-factor authentication electronic lock system, and methods of using the multi-factor authentication electronic lock system. The multi-factor authentication electronic lock includes a user presence detection system, a biometrics authentication system, and a multi-factor authentication electronic lock controller. When a user approaches the multi-factor authentication electronic lock, the user presence detection system detects the presence of user and authenticates the user detected to determine whether the user detected is an authorized user registered. Once the user is determined to be the authorized users registered, the rest of the multi-factor authentication electronic lock including the biometrics authentication system and the multi-factor authentication electronic lock controller is powered on, and biometrics authentication system further authenticates the authorized user detected, and the multi-factor authentication electronic lock is operable only when the authorized user detected is authenticated again by the biometrics authentication system.