Disclosed are a target speaker separation system, an electronic device and a storage medium. The system includes: first, performing, jointly unified modeling on a plurality of cues based a masked pre-training strategy, to boost the inference capability of a model for missing cues and enhance the representation accuracy of disturbed cues; and second, constructing a hierarchical cue modulation module. A spatial cue is introduced into a primary cue modulation module for directional enhancement of a speech of a speaker; in an intermediate cue modulation module, the speech of the speaker is enhanced on the basis of temporal coherence of a dynamic cue and an auditory signal component; a steady-state cue is introduced into an advanced cue modulation module for selective filtering; and finally, the supervised learning capability of simulation data and the unsupervised learning effect of real mixed data are sufficiently utilized.