Systems and methods for continuous real-time sweat sampling and analysis are disclosed. A sweat collection article and method of collecting sweat from a person's skin using the sweat collection article are provided. The sweat collection article includes: a sweat collecting tube placed adjacent to the person's skin, and at least one piece of flexible material that has an adhesive that adheres the article to the person's skin and is positioned to overlie one end of the tube. The second end of the sweat collecting tube is in fluid communication with an instrument such as a mass spectrometer that analyzes the sweat on a real-time basis. The systems and methods may further include a device for removing salt from the sweat that is arranged so that the sweat is transported to the device prior to being transported to the instrument for analyzing the sweat.