Systems and methods for synchronization are provided. In some aspects, a method for synchronizing an image sensor is provided. The method includes receiving image data captured using an image sensor that is moving along a pathway, and assembling an image sensor trajectory using the image data. The method also includes receiving position data acquired along the pathway using a position sensor, wherein timestamps for the image data and position data are asynchronous, and assembling a position sensor trajectory using the position data. The method further includes generating a spatial transformation that aligns the image sensor trajectory and position sensor trajectory, and synchronizing the image sensor based on the spatial transformation.