A display substrate includes a display region and a peripheral region on a periphery of the display region. At least multiple sub-pixels are arranged in the display region. At least one sub-pixel includes a pixel driving circuit arranged on an underlay substrate and a light emitting element connected with the pixel driving circuit. The pixel driving circuit is connected with a first initial signal line. The first initial signal line is arranged to provide a first initial voltage to the light emitting element under the control of the pixel driving circuit. A first power line and a signal connection line are arranged in the peripheral region. The first initial signal line is connected with the first power line through the signal connection line in the peripheral region.