The present disclosure provides use of a Chaetomium globosum strain in controlling Fusarium crown rot (FCR) of wheat. The C. globosum strain under the deposit designation of 12XP1-2-3 is deposited at China General Microbiological Culture Collection Center (CGMCC) on Jan. 23, 2019 with an accession number of CGMCC No. 17183. Field experiments conclude that coating of wheat seeds with Chaetomium globosum strain 12XP1-2-3 can reduce diseased stem rate, disease rating, and disease index by 19.0%-41.3%, 43.7%, and 4.7%-45.4%, respectively.