Invention Grant
- Patent Title: Non-specific nucleases from the genus
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Application No.: US17264748Application Date: 2019-07-26
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Publication No.: US11999980B2Publication Date: 2024-06-04
- Inventor: Skander Elleuche , Sarah Schmitz , Stefan Miltenyi , Volker Nolle
- Applicant: Miltenyi Biotec B.V. & Co. KG
- Applicant Address: DE Bergisch Gladbach
- Assignee: Miltenyi Biotec B.V. & Co. KG
- Current Assignee: Miltenyi Biotec B.V. & Co. KG
- Current Assignee Address: DE Bergisch Gladbach
- Agency: Kilpatrick Townsend & Stockton LLP
- Priority: EP 186232 2018.07.30
- International Application: PCT/EP2019/070148 2019.07.26
- International Announcement: WO2020/025468A 2020.02.06
- Date entered country: 2021-01-29
- Main IPC: C12N9/22
- IPC: C12N9/22 ; C12N9/96 ; G01N1/40
Abstract:
The present invention provides a composition comprising an isolated polypeptide having non-specific nuclease (NSN) activity for degrading nucleic acids comprising or consisting of at least 70% amino acid sequence identity to SEQ ID NO:2, wherein said polypeptide has NSN activity in a solution of about 4° C., and a cation complexing agent such as EDTA. Said composition may have preferentially a neutral to basic pH. A kit comprising said composition and a method using said composition are also disclosed.
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