A disposable self-sensing signal test strip includes a test strip body. The test strip body has a detection area and a circuit area. The detection area has a detection circuit. An electrochemical processing unit, a wireless transmission unit, and a power source unit are provided in the circuit area. The detection circuit is electrically connected to the electrochemical processing unit. The electrochemical processing unit sends to the detection circuit a control signal for performing detection. After receiving the control signal, the detection circuit reacts electrochemically with the test sample and sends a feedback signal to the electrochemical processing unit. The electrochemical processing unit converts the feedback signal into a detection parameter signal and sends the detection parameter signal through the wireless transmission unit to a receiving unit. The power source unit supplies electricity to the electrochemical processing unit and the wireless transmission unit.