The disclosure discloses a display substrate and a display device. The display substrate includes a base substrate, and a first wire layer, a first insulating layer, a transitional connection layer, a second insulating layer, a third insulating layer, and a second wire layer sequentially arranged on a wire changing region of the substrate; first wires of the first wire layer are electrically connected to electrical connection portions of the transitional connection layer through a first opening of the first insulating layer, and the electrical connection portions are electrically connected to second wires of the second wire layer through a second opening of the second insulating layer and a third opening of the third insulating layer; at least two of a projection of the first opening, a projection of the second opening, or a projection of the third opening on the substrate are staggered in positions.