Invention Grant
- Patent Title: Engineered polymerases with reduced sequence-specific errors
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Application No.: US18160951Application Date: 2023-01-27
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Publication No.: US12006518B2Publication Date: 2024-06-11
- Inventor: Jendrik Hentschel , Tyler Lopez , Michael Klein , Virginia Saade , Matthew Kellinger , Mark Ambroso
- Applicant: Element Biosciences, Inc.
- Applicant Address: US CA San Diego
- Assignee: ELEMENT BIOSCIENCES, INC.
- Current Assignee: ELEMENT BIOSCIENCES, INC.
- Current Assignee Address: US CA San Diego
- Agency: Fenwick & West LLP
- Main IPC: C12N9/12
- IPC: C12N9/12 ; C12Q1/6869

Abstract:
Provided herein are engineered variants of archaeal polymerases that exhibit exonuclease-minus activity, enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, improved uracil-tolerance and/or reduce sequence-specific errors in polymerase-catalyzed nucleotide binding and extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases and forming binding complexes, and uses for conducting nucleic acid sequencing reactions.
Public/Granted literature
- US20230265401A1 ENGINEERED POLYMERASES WITH REDUCED SEQUENCE-SPECIFIC ERRORS Public/Granted day:2023-08-24
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