An electronic substrate and an electronic device are provided. The electronic substrate includes a first functional region, a second functional region, and a peripheral region; the first functional region includes an opening, the second functional region is in the opening, and the peripheral region includes an opening peripheral region between the first functional region and the second functional region; the electronic substrate includes a base substrate and a detection trace structure on the base substrate; the detection trace structure includes a first conductive trace and a second conductive trace which are in the opening peripheral region, and the first conductive trace and the second conductive trace respectively extend from a first position reversely along an edge of the second functional region and respectively partially surround the second functional region, the first conductive trace and the second conductive trace are spaced apart from each other in the first position.