A display substrate includes a base substrate, a first conductive pattern, an organic layer and a second conductive layer. A via hole penetrating the organic layer is arranged in the organic layer along a direction perpendicular to the base substrate, a position of the via hole corresponds to a position of the first conductive pattern, and the second conductive layer is electrically connected to the first conductive pattern through the via hole. The display substrate further includes a filling structure for filling the via hole, a distance difference between a distance from a surface of the filling structure on a side away from the base substrate to the base substrate and a distance from a surface of the organic layer on a side away from the base substrate to the base substrate is smaller than a preset threshold.