The disclosure discloses a system and method for predicting a vibration of a bicycle when being rode on a road. The method first uses a pressure film to obtain a contact interface between a road and a bicycle tire, and then, based on the contact interface, a unit bearing area (Bu) and an average stress peak distance (Spa) are calculated. Next, taking the above parameters as variables, a predicted vibration value (Pv) is calculated based on calculation formulas provided by the present disclosure. Finally, the predicted vibration value Pv is compared with a comfort-level threshold perceived by a cyclist of the present disclosure, which can quickly and effectively grade and predict a comfort level when riding a bicycle on a section of an asphalt road.