A self-cleaning filtration media for use in hydrocarbon fluid filtration applications has a first layer on an upstream side and a second layer on downstream side of the self-cleaning filtration media. The first layer is composed of polyethersulfone nanofibers having a diameter of 50-1000 nm (0.05-1 micron) and a basis weight of at least 1 gsm. The second layer is a nonwoven substrate suitable for filtration applications. The self-cleaning filtration media exhibits a dust holding capacity of at least 6 mg/cm2 and a filtration efficiency of greater than 90% for 4 micron particles when the self-cleaning filtration media is tested according to ISO 19438 for fuel filtration and according to ISO 4548-12 for oil filtration. When the second layer includes glass fibers, the self-cleaning filtration media has a dust holding capacity of at least 10 mg/cm2 and a filtration efficiency of greater than 99% for 4 micron particles when the self-cleaning filtration media is tested according to ISO 19438 for fuel filtration and according to ISO 4548-12 for oil filtration. The self-cleaning filtration media also has a fuel-water separation efficiency of at least 99% when a flat sheet is tested according to ISO 16332; and a lifetime of at least 90 minutes when tested with fuel and oil according to ISO 19438 and ISO 4548-12 using Medium Test Dust and a pressure drop of 70 kPa.