Invention Grant
- Patent Title: RNASE H2 mutants that reduce primer dimers and off-target amplification in RHPCR-based amplicon sequencing with high-fidelity DNA polymerases
-
Application No.: US17558994Application Date: 2021-12-22
-
Publication No.: US12024742B2Publication Date: 2024-07-02
- Inventor: Joseph Dobosy , John Froehlig, Jr. , Katherine Perschbacher , Kristin Beltz , Scott Rose , Mark Aaron Behlke
- Applicant: Integrated DNA Technologies, Inc.
- Applicant Address: US IA Coralville
- Assignee: Integrated DNA Technologies, Inc.
- Current Assignee: Integrated DNA Technologies, Inc.
- Current Assignee Address: US IA Coralville
- Agency: Klintworth & Rozenblat IP LLP
- Main IPC: C12Q1/6848
- IPC: C12Q1/6848 ; C12N9/22 ; C12Q1/6853 ; C12Q1/686
Abstract:
The present invention pertains to hybrid RNase H2 proteins that include fragments of amino acid sequences from Pyrococcus abyssi (P.a.), Thermococcus kodakarensis (T.kod), and Pyrococcus furiosus organisms, as well as methods of using the same to improve mismatch discrimination and activity in a high-fidelity DNA polymerase buffer.
Public/Granted literature
Information query
