A durability evaluation method of a secondary battery includes a durability step of continuing to apply an alternating current voltage of a durability frequency to a first secondary battery for evaluation over a durability test period, and an evaluation step of evaluating durability of an associated battery reaction associated with the durability frequency from among battery reactions of the first secondary battery. A temperature and a state of charge of the first secondary battery are adjusted to a first temperature and a first state of charge, respectively.