A display device includes a first substrate, a first alignment layer, a second substrate, a second alignment layer, and a display medium. The first alignment layer is disposed on the first substrate. The second substrate is disposed opposite to the first substrate. The second alignment layer is disposed on the second substrate, and the first and second alignment layers are located between the first and second substrates. The display medium is located between the first and second alignment layers, where a surface of the first alignment layer facing the display medium and a surface of the second alignment layer facing the display medium have topographies with irregular areas surrounded by micro-structures. Besides, a manufacturing method of the display device is also provided.