A novel light-emitting device is provided. Alternatively, a light-emitting device with favorable emission efficiency is provided. Alternatively, a light-emitting device with a favorable lifetime is provided. Alternatively, a light-emitting device with a low driving voltage is provided. Provided is a light-emitting device including an anode, a cathode, and a layer including an organic compound that is positioned between the anode and the cathode, in which the layer including the organic compound includes a first layer, a second layer, and a light-emitting layer in this order from the anode side, the first layer includes a first substance and a second substance, the second layer includes a third substance, the first substance is an organic compound a HOMO level of which is higher than or equal to −5.8 eV and lower than or equal to −5.4 eV, the second substance is a substance that has an electron-acceptor property with respect to the first substance, and the third substance is an organic compound having a structure in which at least two substituents comprising carbazole rings are bonded to a naphthalene ring.