An aneuploid Stevia cultivar from colchicine-induced polyploidy, designated ‘AP-1’. The plant parts of Stevia cultivar ‘AP-1’, the plants of stevia ‘AP-1’ and methods for producing a Stevia plant produced by crossing the cultivar ‘AP-1’ with itself or another Stevia variety, including methods using colchicine treatments for producing an aneuploidy Stevia plant. The disclosure further relates to hybrid Stevia seeds and plants produced by crossing the cultivar ‘AP-1’ with another Stevia cultivar. Ten highly polymorphic SNPs and the corresponding genomic sequences used to identify ‘AP-1’.