Provided is an optically anisotropic film, an optical film, and a display device, which have a high contrast and excellent light fastness. The optically anisotropic film includes a liquid crystal compound and a dichroic substance, in which the liquid crystal compound is vertically aligned, the dichroic substance forms an arrangement structure, and in a case where, in a cross section observed with a scanning transmission electron microscope, a length of a major axis of the arrangement structure is defined as L, and a length of a minor axis of the arrangement structure is defined as D, 16 or more arrangement structures satisfying L≥30 nm are observed per 40 μm2.