Invention Grant
- Patent Title: PfAgo mutant protein mesophilic target nucleic acid cleavage activity and application thereof
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Application No.: US18424759Application Date: 2024-01-27
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Publication No.: US12054757B1Publication Date: 2024-08-06
- Inventor: Lixin Ma , Longyu Wang , Fei Wang , Xiaochen Xie , Wanping Chen , Fuyong Huang
- Applicant: Hubei University
- Applicant Address: CN Wuhan
- Assignee: HUBEI UNIVERSITY
- Current Assignee: HUBEI UNIVERSITY
- Current Assignee Address: CN Wuhan
- Agent Zhigang Ma
- Priority: CN 2310562239.7 2023.05.16
- Main IPC: C12N9/22
- IPC: C12N9/22 ; C12N15/11 ; C12N15/90 ; C12Q1/6823
Abstract:
A Pyrococcus furiosus Argonaute (PfAgo) mutant protein with mesophilic target nucleic acid cleavage activity and an application thereof are provided. The PfAgo mutant protein has an amino acid mutation on at least one of a 617th position and a 618th position relative to the sequence shown in SEQ ID NO: 1, specifically, K617E/G and/or L618Y/F/W/G. Compared with a wild-type PfAgo protein, the provided PfAgo mutant protein has activity in the temperature range of 30-95 Celsius degree (° C.), effectively expanding application range of prokaryotic Argonaute (pAgo) protein.
Information query
