Invention Grant
- Patent Title: High-efficiency synthesis and high-purity hyaluronic acid, and recombinant
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Application No.: US17755191Application Date: 2019-12-17
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Publication No.: US12054760B2Publication Date: 2024-08-06
- Inventor: Zhen Kang , Jian Chen , Litao Hu , Guocheng Du , Yang Wang , Jianghua Li , Jialian Li , Tianmeng Zhang
- Applicant: BLOOMAGE BIOTECHNOLOGY CORPORATION LIMITED , JIANGNAN UNIVERSITY
- Applicant Address: CN Jinan
- Assignee: BLOOMAGE BIOTECHNOLOGY CORPORATION LIMITED,JIANGNAN UNIVERSITY
- Current Assignee: BLOOMAGE BIOTECHNOLOGY CORPORATION LIMITED,JIANGNAN UNIVERSITY
- Current Assignee Address: CN Jinan; CN Wuxi
- Agency: Hoxie & Associates LLC
- Priority: CN 1911018182.4 2019.10.24
- International Application: PCT/CN2019/126013 2019.12.17
- International Announcement: WO2021/077580A 2021.04.29
- Date entered country: 2022-04-22
- Main IPC: C12P19/04
- IPC: C12P19/04 ; C12N1/20 ; C12N9/10 ; C12N9/26 ; C12N15/77 ; C12R1/15

Abstract:
The invention discloses a recombinant Corynebacterium glutamicum for efficient synthesis of highly pure hyaluronic acid and oligosaccharides thereof, belonging to the technical field of bioengineering. The recombinant Corynebacterium glutamicum constructed in the present invention can produce hyaluronic acid with a yield up to 40 g/L, and a crude product purity of 95%. Addition of exogenous hyaluronic acid hydrolase and optimization of the fermentation conditions results in hyaluronic acid oligosaccharides with specific molecular weight, and can further improve the yield of hyaluronic acid to 72 g/L. The invention lays a solid foundation for the efficient synthesis of highly pure hyaluronic acid by microorganisms, and the constructed recombinant Corynebacterium glutamicum is suitable for industrial production and application.
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