Methods, systems, and computer readable media for test system agent deployment in a smartswitch computing environment are disclosed. One example method occurs at a test system, the method comprising: obtaining test information indicating a smartswitch or a data processing unit (DPU) of the smartswitch for testing in a smartswitch computing environment or at least one test system agent deployment factor, wherein the smartswitch includes a switch processor and DPUs; determining, using the test information and topology information about the smartswitch computing environment, at least one location for deploying at least one test system agent; and providing placement information indicating the at least one location for deploying the at least one test system agent to a target system or to a test system agent controller.