The display substrate includes a base substrate and a plurality of pixel units disposed on the base substrate. Each of the pixel units is composed of 5 sub-pixels. The 5 sub-pixels include 1 red sub-pixel, 2 green sub-pixels, 1 blue sub-pixel and 1 white sub-pixel. Each of the sub-pixels includes a white light emitting unit. The red sub-pixel, the green sub-pixels and the blue sub-pixel further include color film layers corresponding to respective light emitting colors of the red sub-pixel, the green sub-pixels and the blue sub-pixel. The white sub-pixel is adjacent to the blue sub-pixel, and an aperture area of the blue sub-pixel is smaller than that of the green sub-pixels and smaller than that of the red sub-pixel.