Optical fiber mass splice methods and assemblies are provided. A method may include securing a fiber clamp to a fiber setting fixture, the fiber setting fixture including a fiber alignment block and a backstop. A plurality of fiber grooves may be defined in the fiber alignment block. The method may further include inserting a plurality of optical fibers into the fiber setting fixture such that each of the plurality of optical fibers is disposed in one of the plurality of fiber grooves and contacts the backstop. The method may further include loading, after the inserting step, each of the plurality of optical fibers into the fiber clamp. The method may further include clamping the plurality of optical fibers in the fiber clamp.