Methods of calibrating a light-field imaging system, a light-field imaging system to perform the calibration methods, a calibration target for the calibration methods, and methods of projecting a light-field image into object space with a calibrated light-field imaging system. An exemplary calibration method is performed with a light-field imaging system including a microlens array (72) and an image sensor (76). A z-stack of light-field images of a calibration target may be captured using the image sensor, while the calibration target is on the stage and located at a plurality of different z-positions. A total magnification of the imaging system and a microlens magnification of the microlens array may be determined from each light-field image of the z-stack.