A driving circuit of fluid pump module includes a microprocessor, a primary boost circuit, and a pump driving circuit is provided. The microprocessor receives an output signal with a large-width variable rectangular waveform, a driving voltage, a first detection current-feedback signal, and a second detection current-feedback signal. The primary boost circuit converts an inputted driving voltage into a direct current with a certain high voltage. The pump driving circuit receives the certain high voltage and is connected with the microprocessor to receive the voltage control signal and the pulse-width modulation (PWM) signal. The secondary boost circuit receives the certain high voltage to boost the certain high voltage into a working voltage for the fluid pump. The operation driving circuit receives the working voltage and provides the pulse-width modulation signal for the fluid pump through the second detection current-feedback signal.