A display substrate includes a plurality of sub-pixels, wherein at least one sub-pixel includes a driving circuit layer and a light emitting structure layer disposed on the driving circuit layer; the driving circuit layer includes a pixel driving circuit, and the light emitting structure layer includes a light emitting device connected with the pixel driving circuit; the pixel driving circuit includes a reset sub-circuit, a writing sub-circuit, a driving sub-circuit, a compensation sub-circuit, an energy storage sub-circuit and a light emitting control sub-circuit; and at least one of the reset sub-circuit, the compensation sub-circuit, and the writing sub-circuit includes an oxide transistor and a one-way conductive device.