A portable negative air pressure apparatus for isolating a treatment subject to permit a selected treatment procedure to be performed by a treatment operator. The apparatus comprises an air source connected to a closed chamber for sealable disposition over the head, neck and upper thorax of the treatment subject with the chamber having air ports, in and out. The chamber has rigid clear sides and a plurality of sealed ports, with the ports including sealed armholes for access, and with sealed gloves connected to the sealed armholes. The chamber also has at least one instrument hatch to permit introduction into the chamber of instruments for carrying-out the selected treatment procedure.