Invention Grant
- Patent Title: Method for super-resolution evaluation of microscope images illuminated in a structured manner and microscope having structured illumination
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Application No.: US17470099Application Date: 2021-09-09
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Publication No.: US12094081B2Publication Date: 2024-09-17
- Inventor: Ingo Kleppe , Yauheni Novikau
- Applicant: Carl Zeiss Microscopy GmbH
- Applicant Address: DE Jena
- Assignee: Carl Zeiss Microscopy GmbH
- Current Assignee: Carl Zeiss Microscopy GmbH
- Current Assignee Address: DE Jena
- Agency: Duane Morris LLP
- Priority: DE 2020211380.7 2020.09.10
- Main IPC: G06T3/40
- IPC: G06T3/40 ; G02B21/36 ; G06T3/4053 ; G06T5/20 ; H04N23/56 ; H04N23/951

Abstract:
Method for super-resolution evaluation of microscope images illuminated in a structured manner and microscope having structured illumination. The resolution can be improved laterally by a factor of up to two using conventional linear structured Illumination (SIM). If a non-linear iterative method is used for the purpose of deconvolution, the achievable resolution can be improved beyond the theoretical limit. However, the known methods only achieve a small amount of increase. The novel method is intended to make improved resolution or improved contrast possible. If a PSF/OTF that is manipulated (individually for each order) in the same (or in a corresponding) way as the relevant order spatial frequency spectrum is used during the re-weighting in the spatial frequency domain (for the deconvolution), the actually achievable resolution can be nearly doubled in comparison with the conventional SIM, both in one-stage and in two-stage variants.
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