Provided are an array substrate, a manufacturing method thereof, and a display panel. The array substrate includes: a substrate, a first active layer of a first thin film transistor, a first insulating layer, a first metal layer, and a second active layer of a second thin film transistor. The first metal layer includes a first connection portion, which overlaps one of a source contact region or a drain contact region of the first active layer and overlaps one of a source contact region or a drain contact region of the second active layer. The one of the source contact region or the drain contact region of the first active layer and the one of the source contact region or the drain contact region of the second active layer overlap each other, and are electrically connected through a via in the first insulating layer and the first connection portion.