A display substrate and a display device are disclosed. The display substrate includes a base substrate, a pixel circuit layer, and an anode layer; the pixel circuit layer includes a plurality of pixel driving circuits; and the anode layer includes a plurality of anode; each pixel driving circuit includes a functional thin film transistor; the plurality of pixel driving circuits include a first pixel driving circuit and a second pixel driving circuit which are adjacently arranged, and an orthographic projection of a channel region of the functional thin film transistor in the first pixel driving circuit on the base substrate and an orthographic projection of a channel region of the functional thin film transistor in the second pixel driving circuit on the base substrate both overlap with an orthographic projection of the anode corresponding to the first pixel driving circuit on the base substrate.