According to the present disclosure, there is provided a cell stimulation and culture platform using a ultrasonic hologram, including a culture vessel in which a culture well with an open lower portion is formed; a transmission sheet which is installed to cover a lower surface of the culture vessel and where a biological sample is seated; a platform body that is filled with a liquid medium and an open upper end is covered by the transmission sheet; an ultrasonic transducer that is installed inside the platform body in a state of being spaced apart from the biological sample; and an ultrasonic hologram lens that is installed on the ultrasonic transducer, and spatially modulates the phase of the ultrasonic waves using a surface structure designed to have different height distributions to focus the ultrasonic waves in a set pattern shape on a target surface on which the biological sample is located.