A glycosyltransferase BS-YjiC mutant, a construction method and an application thereof provided, relating to the field of genetic engineering technologies. The glycosyltransferase BS-YjiC mutant is obtained by performing site-directed mutation on amino acids at the 125th, 178th, 313th, 125th and 178th, or 125th and 313th positions of a wild-type glycosyltransferase BS-YjiC with the amino acid sequence as shown in SEQ ID NO: 1. Compared with the wild-type glycosyltransferase BS-YjiC, the glycosyltransferase BS-YjiC mutant is more suitable for catalyzing protopanaxadiol (PPD) to generate rare ginsenosides F12 and Rh2, which is more conducive to the flexibility of the production process.