Provided are a display substrate, a preparation method thereof, and a display apparatus. The display substrate includes: a first display region and a second display region; the first display region includes a plurality of first pixels, wherein the first pixel includes a passive light-emitting device, the second display region includes a plurality of second pixels, wherein the second pixel includes an active light-emitting device and a pixel drive circuit electrically connected to the active light-emitting device. The first display region includes a plurality of first driving signal wire groups, each first driving signal wire group corresponds to a first pixel row. The first pixel row includes one row of the first pixels, and each first driving signal group includes a plurality of first driving signal wires.