Methods to improve site-directed integration frequency

Invention Grant

US12157893B2 Methods to improve site-directed integration frequency 有权

Patent Title: Methods to improve site-directed integration frequency
Application No.: US17523755

Application Date: 2021-11-10
Publication No.: US12157893B2

Publication Date: 2024-12-03
Inventor: John Bradley , Larry A. Gilbertson , Ervin Nagy , Christine Shyu , Peizhen Yang , Xudong Ye
Applicant: MONSANTO TECHNOLOGY LLC
Applicant Address: US MO St. Louis
Assignee: MONSANTO TECHNOLOGY LLC
Current Assignee: MONSANTO TECHNOLOGY LLC
Current Assignee Address: US MO St. Louis
Agency: Arnold & Porter Kaye Scholer LLP
Main IPC: C12N15/82
IPC: C12N15/82

Abstract:

The present disclosure relates to compositions and methods for improving site-directed integration frequency in the genome of plant cells.

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IPC分类:

C	化学；冶金
C12	生物化学；啤酒；烈性酒；果汁酒；醋；微生物学；酶学；突变或遗传工程
C12N	微生物或酶；其组合物；繁殖、保藏或维持微生物；变异或遗传工程；培养基（微生物学的试验介质入C12Q1/00）。
C12N15/00	突变或遗传工程；遗传工程涉及的DNA或RNA，载体（如质粒）或其分离、制备或纯化；所使用的宿主（突变体或遗传工程制备的微生物本身入C12N1/00、C12N5/00、C12N7/00；新的植物入A01H；用组织培养技术再生植物入A01H4/00；新的动物入A01K67/00；含有插入活体细胞的遗传物质以治疗遗传疾病的药剂的应用，基因疗法入A61K48/00，一般肽入C07K）
C12N15/09	.DNA重组技术
C12N15/63	..使用载体引入外来遗传物质；载体；其宿主的使用；表达的调节
C12N15/79	...专门适用于真核细胞宿主的载体或表达系统
C12N15/82	....用于植物细胞