A forming method of a semiconductor structure includes the following: providing a semiconductor substrate formed with a first mask layer having a preset pattern; forming a second mask layer having a first mask pattern on a surface of the first mask layer, wherein the first mask pattern includes a plurality of first sub-patterns arranged in sequence; forming a second mask pattern in the second mask layer through the first mask pattern in a self-alignment manner, wherein the second mask pattern includes the first sub-patterns of the first mask pattern and second sub-patterns corresponding to the first sub-patterns; etching the first mask layer based on the first sub-patterns and the second sub-patterns of the second mask pattern to convert the preset pattern into an active area pattern; and defining active areas in the semiconductor substrate based on the active area pattern.