Various embodiments are directed to a tote stop assembly configured for a conveyor assembly and methods of operating the same. In various embodiments, the tote stop assembly comprises a cam assembly comprising a cam element secured relative to an outer surface of a drive roller, the drive roller being configured to cause motor-driven rotation of the cam element about a central axis of the drive roller; and a tote stop component comprising a cam follower configured for physical engagement with the cam element such that the motor-driven rotation of the cam element causes vertical movement of the tote stop component between raised and lowered positions; the tote stop assembly being configurable in an actuated configuration to selectively obstruct an object from moving along a conveyor path based on an operation of the drive roller, the actuated configuration being defined by the tote stop component being arranged in the raised position.