An apparatus for determining defective hair follicles includes an image acquiring unit for acquiring an image of a follicle and a hair for each follicle separated from a scalp of an alopecic patient in an incisional hair transplant or each follicle directly extracted from an alopecic patient in a non-incisional hair transplant, an image processing unit for extracting an outline pattern of the image of the follicle and the hair by performing a contour detection process or an edge detection process on the image, a follicle shape database for storing hair pixel patterns related to various shapes of hairs and follicle pixel patterns related to various shapes of follicles, and a follicle determining unit for determining whether a follicle is normal follicle or defective follicle by comparing the outline pattern of the image with the hair pixel patterns and follicle pixel patterns stored in the follicle shape database.