A display substrate, includes: a display region. The display region includes a plurality of sub-pixels disposed on a substrate, a plurality of first signal lines extending in a first direction, and a plurality of data lines extending in a second direction. At least one sub-pixel includes a driving circuit, and the driving circuit includes a plurality of transistors and at least one storage capacitor. The transistor at least includes a first conductive layer and a second conductive layer. The plurality of first signal lines is located in a third conductive layer. The third conductive layer is located on a side of a control electrode of a transistor of the driving circuit away from the substrate.