A display substrate and a display device. The display substrate includes: a plurality of pixel islands, a first opening, a second opening and a first passage region. Each pixel island includes at least one pixel, each pixel includes a plurality of first driving lines, the first passage region is provided with a plurality of first connection lines, each of the plurality of pixel islands further includes a plurality of transfer lines, and the plurality of transfer lines are arranged in different layers from the plurality of first driving lines and cross each other to form a plurality of overlapping regions; the plurality of transfer lines are electrically connected with the plurality of first driving lines through via holes located in part of the overlapping regions, and the transfer lines in two adjacent pixel islands are respectively connected with the plurality of first connection lines in the first passage region.