A triboelectric nanogenerator (TENG) using a 3D-spacer fabric and polydimethylsiloxane (PDMS) shows great application potential for biokinetic energy harvesting and a multifunctional self-power device. In the present disclosure, a TENG with a fabric-PDMS-fabric structure is fabricated using diverse three-dimensional (3D) fabrics and PDMS. Peak-to-peak output voltages of the diverse 3D-spacer fabrics are compared. The output voltages are changed due to structures and vertical fibers. In addition, a coefficient of surface friction between PDMS and fabric improves the output voltage. TENGs using different 3D-spacer polymeric fabrics show different maximum peak-to-peak output voltage performances. This is due to the stiffness, lateral elasticity, and 3D morphology of the fabrics. It is considered that those factors including the stiffness, the lateral elasticity, and the 3D morphology influence the densities in vertical and lateral fiber-to-fiber interaction.