Examples of monitoring of follicular fluid flow using image processing are described herein. Image frames pertaining to a pre-defined target area defined for a collection tube during an ovum pick-up (OPU) step of an in-vitro fertilization (IVF) procedure may be obtained in real-time. The target area corresponds to a region indicating entry of a follicular fluid into the collection tube. Thereafter, the image frames may be processed to detect occurrence of a flow of the follicular fluid as well as presence of blood in the follicular fluid. Accordingly, a first alert signal indicating detection of occurrence of flow of the follicular fluid and a second alert signal indicating presence of blood in the follicular fluid, may be generated to notify a medical practitioner.