A display substrate, including a base substrate, and a driving circuit layer and light emitting elements formed on the base substrate, and in a same pixel circuit, an active general layer includes a first column-wise active portion including active layers of a data writing transistor and a first light emission control transistor, and a first active connection portion, an orthographic projection of the first active connection portion on the base substrate at least partially overlapping an orthographic projection of a corresponding power supply line on the base substrate, a dimension of the first active connection portion in a row direction of the pixel units less than a dimension of the active layer of the data writing transistor in the row direction, and a dimension of the active layer of the first light emission control transistor in the row direction. A display panel is further provided.